Landsat Program

Landsat initiated the revolution in moderate resolution Earth remote sensing in the 1970s. With seven successful missions over 40+ years, Landsat has documented - and continues to document - the global Earth land surface and its evolution. The Landsat missions and sensors have evolved along with the technology from a demonstration project in the analog world of visual interpretation to an operational mission in the digital world, with incremental improvements along the way in terms of spectral, spatial, radiometric and geometric performance as well as acquisition strategy, data availability, and products

Characterization of the Biosynthesis, Processing and Kinetic Mechanism of Action of the Enzyme Deficient in Mucopolysaccharidosis IIIC

Heparin acetyl-CoA:alpha-glucosaminide N-acetyltransferase (N-acetyltransferase, EC 2.3.1.78) is an integral lysosomal membrane protein containing 11 transmembrane domains, encoded by the HGSNAT gene. Deficiencies of N-acetyltransferase lead to mucopolysaccharidosis IIIC. We demonstrate that contrary to a previous report, the N-acetyltransferase signal peptide is co-translationally cleaved and that this event is required for its intracellular transport to the lysosome. While we confirm that the N-acetyltransferase precursor polypeptide is processed in the lysosome into a small amino-terminal alpha- and a larger ß- chain, we further characterize this event by identifying the mature amino-terminus of each chain. We also demonstrate this processing step(s) is not, as previously reported, needed to produce a functional transferase, i.e., the precursor is active. We next optimize the biochemical assay procedure so that it remains linear as N-acetyltransferase is purified or protein-extracts containing N-acetyltransferase are diluted, by the inclusion of negatively charged lipids. We then use this assay to demonstrate that the purified single N-acetyltransferase protein is both necessary and sufficient to express transferase activity, and that N-acetyltransferase functions as a monomer. Finally, the kinetic mechanism of action of purified N-acetyltransferase was evaluated and found to be a random sequential mechanism involving the formation of a ternary complex with its two substrates; i.e., N-acetyltransferase does not operate through a ping-pong mechanism as previously reported. We confirm this conclusion by demonstrating experimentally that no acetylated enzyme intermediate is formed during the reaction

Epidemiology and heritability of Major Depressive Disorder, stratified by age of onset, sex, and illness course in Generation Scotland:Scottish Family Health Study (GS:SFHS)

The heritability of Major Depressive Disorder (MDD) has been estimated at 37% based largely on twin studies that rely on contested assumptions. More recently, the heritability of MDD has been estimated on large populations from registries such as the Swedish, Finnish, and Chinese cohorts. Family-based designs utilise a number of different relationships and provide an alternative means of estimating heritability. Generation Scotland: Scottish Family Health Study (GS:SFHS) is a large (n = 20,198), family-based population study designed to identify the genetic determinants of common diseases, including Major Depressive Disorder. Two thousand seven hundred and six individuals were SCID diagnosed with MDD, 13.5% of the cohort, from which we inferred a population prevalence of 12.2% (95% credible interval: 11.4% to 13.1%). Increased risk of MDD was associated with being female, unemployed due to a disability, current smokers, former drinkers, and living in areas of greater social deprivation. The heritability of MDD in GS:SFHS was between 28% and 44%, estimated from a pedigree model. The genetic correlation of MDD between sexes, age of onset, and illness course were examined and showed strong genetic correlations. The genetic correlation between males and females with MDD was 0.75 (0.43 to 0.99); between earlier (≤ age 40) and later (> age 40) onset was 0.85 (0.66 to 0.98); and between single and recurrent episodic illness course was 0.87 (0.72 to 0.98). We found that the heritability of recurrent MDD illness course was significantly greater than the heritability of single MDD illness course. The study confirms a moderate genetic contribution to depression, with a small contribution of the common family environment (variance proportion = 0.07, CI: 0.01 to 0.15), and supports the relationship of MDD with previously identified risk factors. This study did not find robust support for genetic differences in MDD due to sex, age of onset, or illness course. However, we found an intriguing difference in heritability between recurrent and single MDD illness course. These findings establish GS:SFHS as a valuable cohort for the genetic investigation of MDD

Genome-wide meta-analysis of 241,258 adults accounting for smoking behaviour identifies novel loci for obesity traits

Few genome-wide association studies (GWAS) account for environmental exposures, like smoking, potentially impacting the overall trait variance when investigating the genetic contribution to obesity-related traits. Here, we use GWAS data from 51,080 current smokers and 190,178 nonsmokers (87% European descent) to identify loci influencing BMI and central adiposity, measured as waist circumference and waist-to-hip ratio both adjusted for BMI. We identify 23 novel genetic loci, and 9 loci with convincing evidence of gene-smoking interaction (GxSMK) on obesity-related traits. We show consistent direction of effect for all identified loci and significance for 18 novel and for 5 interaction loci in an independent study sample. These loci highlight novel biological functions, including response to oxidative stress, addictive behaviour, and regulatory functions emphasizing the importance of accounting for environment in genetic analyses. Our results suggest that tobacco smoking may alter the genetic susceptibility to overall adiposity and body fat distribution.Peer reviewe

Genetic stratification of depression by neuroticism: revisiting a diagnostic tradition

Background. Major depressive disorder and neuroticism share a large genetic basis. We sought to determine whether this shared basis could be decomposed to identify genetic factors that are specific to depression. Methods. We analysed summary statistics from genome-wide association studies of depression (from the Psychiatric Genomics Consortium, 23andMe, and UK Biobank) and compared them to genome-wide association studies (GWAS) of neuroticism (from UK Biobank). First, we used a pairwise GWAS analysis to classify variants as associated with only depression, with only neuroticism, or with both. Second, we estimated partial genetic correlations to test whether the depression’s genetic link with other phenotypes was explained by shared overlap with neuroticism. Results. We found evidence that most genomic regions (25/37) associated with depression are likely to be shared with neuroticism. The overlapping common genetic variance of depression and neuroticism was genetically correlated primarily with psychiatric disorders. We found that the genetic contributions to depression, that was not shared with neuroticism, was positively correlated with metabolic phenotypes and cardiovascular disease, and negatively correlated with the personality trait conscientiousness. After removing shared genetic overlap with neuroticism, depression still had a specific association with schizophrenia, bipolar disorder, coronary artery disease, and age of first birth. Independent of depression, neuroticism had specific genetic correlates in ulcerative colitis, pubertal growth, anorexia, and education. Conclusion. Our findings demonstrate that, while genetic risk factors for depression are largely shared with neuroticism, there are also non-neuroticism related features of depression that may be useful for further patient or phenotypic stratification

Re-evaluation of the latent structure of common childhood disorders: is there a general psychopathology factor (P-factor)?

In the field of psychopathology, there is high comorbidity between different disorders. Traditionally, support for two broad correlated dimensions of internalizing and externalizing symptoms has consistently emerged for children and adolescents. To date, oblique 2 and 3 first-order factor models (factors for externalizing and internalizing, and fear, distress, and externalizing) and bi-factor models with the corresponding two and three group factors have been suggested for common internalizing and eternalizing child and adolescent disorders. The present study used confirmatory factor analyses to examine the relative support for these models in adolescents (≥ 12 to 18 years; N = 866) and children (6 to < 12 years; N = 1233) and the reliability and convergent and divergent validities of the psychopathology factor (P-factor) and group factors in the optimum bi-factor model. All participants were from a clinic and underwent Diagnostic and Statistical Manual of Mental Disorders, 4th Edition clinical diagnosis. The findings showed that the bi-factor model with two group factors (internalizing and externalizing) was the optimum model for both children and adolescents. For both groups, findings showed relatively higher reliability for the P-factor than the group factors, although the externalizing group factor showed substantial reliability in adolescents, and both the externalizing and internalizing group factors also showed substantial reliability in children. The factors of the optimum bi-factor model also showed good convergent and discriminant validities. The implications for theory and clinical and research practice related to psychopathology are discussed

Landsat 9 Geometric Characteristics Using Underfly Data

The Landsat program has a long history of providing remotely sensed data to the user community. This history is being extended with the addition of the Landsat 9 satellite, which closely mimics the Landsat 8 satellite and its instruments. These satellites contain two instruments, the Operational Land Imager (OLI) and the Thermal Infrared Sensor (TIRS). OLI is a push-broom sensor that collects visible and near-infrared (VNIR) and short-wave infrared (SWIR) wavelengths at 30 m ground sample distance, along with a panchromatic 15 m band. The TIRS sensor contains two long-wave thermal spectral channels centered at 10.9 and 12 &micro;m. The data from these two instruments, on both satellites, are combined into a single Landsat product. The Landsat 5&ndash;9 satellites follow a 16 day repeat cycle designated as the Worldwide Reference System (WRS-2), which provides a global notional gridded mapping for identifying individual Landsat scenes. The Landsat 8 and 9 satellites are flown such that their orbital tracks are separated by 8 days in this 16 day cycle. During the commissioning period of Landsat 9, and during its ascent to its operational WRS-2 orbit, the Landsat 9 satellite&rsquo;s orbital track went under and crossed over the orbital track of the Landsat 8 satellite. This produced a unique situation where nearly time-coincident imagery could be obtained from the instruments of the two spacecrafts. From a radiometric standpoint, this allowed for near-time cross-calibration between the instruments to be performed. From a geometry perspective, calibration is achieved through high-resolution reference imagery over specific ground locations, thus ensuring calibration of the instruments and for the instruments to be well cross-calibrated geometrically. Although these underfly data do not provide calibration of the instruments between the platforms from a geometric perspective, they allow for the verification of the calibration steps involving the instruments and spacecraft. This paper discusses the co-registration of this unique set of data while also discussing other geometric aspects of these data by looking at and comparing the differences in sensor viewing and sun angles associated with the collections from the two platforms for imagery obtained over common geographic locations. The image-to-image comparisons between Landsat 8 and 9 coincident pairs, where both datasets are precision terrain products, are registered to within 2.2 m with respect to their root-mean-squared radial error (RMSEr). The 2.2 m represents less than 0.1 of a 30 m multispectral pixel in misregistration between the L9 and L8 underfly products that will be available to the user community. This unique dataset will provide well-registered, near-coincident image acquisitions between the two platforms that can be a key to any calibration or application comparisons. The paper also presents that, for images for which one of the image pairs failed precision corrections and became a terrain-corrected only product type, a range of 8&ndash;14 m RMSEr could be expected in co-registration, while, in cases where both image pairs failed the precision correction step and both images became a terrain-corrected only product type, a 14 m RMSEr could be expected for co-registration

Landsat 9 Geometric Characteristics Using Underfly Data

The Landsat program has a long history of providing remotely sensed data to the user community. This history is being extended with the addition of the Landsat 9 satellite, which closely mimics the Landsat 8 satellite and its instruments. These satellites contain two instruments, the Operational Land Imager (OLI) and the Thermal Infrared Sensor (TIRS). OLI is a push-broom sensor that collects visible and near-infrared (VNIR) and short-wave infrared (SWIR) wavelengths at 30 m ground sample distance, along with a panchromatic 15 m band. The TIRS sensor contains two long-wave thermal spectral channels centered at 10.9 and 12 µm. The data from these two instruments, on both satellites, are combined into a single Landsat product. The Landsat 5–9 satellites follow a 16 day repeat cycle designated as the Worldwide Reference System (WRS-2), which provides a global notional gridded mapping for identifying individual Landsat scenes. The Landsat 8 and 9 satellites are flown such that their orbital tracks are separated by 8 days in this 16 day cycle. During the commissioning period of Landsat 9, and during its ascent to its operational WRS-2 orbit, the Landsat 9 satellite’s orbital track went under and crossed over the orbital track of the Landsat 8 satellite. This produced a unique situation where nearly time-coincident imagery could be obtained from the instruments of the two spacecrafts. From a radiometric standpoint, this allowed for near-time cross-calibration between the instruments to be performed. From a geometry perspective, calibration is achieved through high-resolution reference imagery over specific ground locations, thus ensuring calibration of the instruments and for the instruments to be well cross-calibrated geometrically. Although these underfly data do not provide calibration of the instruments between the platforms from a geometric perspective, they allow for the verification of the calibration steps involving the instruments and spacecraft. This paper discusses the co-registration of this unique set of data while also discussing other geometric aspects of these data by looking at and comparing the differences in sensor viewing and sun angles associated with the collections from the two platforms for imagery obtained over common geographic locations. The image-to-image comparisons between Landsat 8 and 9 coincident pairs, where both datasets are precision terrain products, are registered to within 2.2 m with respect to their root-mean-squared radial error (RMSEr). The 2.2 m represents less than 0.1 of a 30 m multispectral pixel in misregistration between the L9 and L8 underfly products that will be available to the user community. This unique dataset will provide well-registered, near-coincident image acquisitions between the two platforms that can be a key to any calibration or application comparisons. The paper also presents that, for images for which one of the image pairs failed precision corrections and became a terrain-corrected only product type, a range of 8–14 m RMSEr could be expected in co-registration, while, in cases where both image pairs failed the precision correction step and both images became a terrain-corrected only product type, a 14 m RMSEr could be expected for co-registration

Current self-reported symptoms of attention deficit/hyperactivity disorder are associated with total brain volume in healthy adults.

Contains fulltext : 110756.pdf (publisher's version ) (Open Access)BACKGROUND: Reduced total brain volume is a consistent finding in children with Attention Deficit/Hyperactivity Disorder (ADHD). In order to get a better understanding of the neurobiology of ADHD, we take the first step in studying the dimensionality of current self-reported adult ADHD symptoms, by looking at its relation with total brain volume. METHODOLOGY/PRINCIPAL FINDINGS: In a sample of 652 highly educated adults, the association between total brain volume, assessed with magnetic resonance imaging, and current number of self-reported ADHD symptoms was studied. The results showed an association between these self-reported ADHD symptoms and total brain volume. Post-hoc analysis revealed that the symptom domain of inattention had the strongest association with total brain volume. In addition, the threshold for impairment coincides with the threshold for brain volume reduction. CONCLUSIONS/SIGNIFICANCE: This finding improves our understanding of the biological substrates of self-reported ADHD symptoms, and suggests total brain volume as a target intermediate phenotype for future gene-finding in ADHD